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![](../images/sub_01_01.png)
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![](http://vic21mr.cafe24.com/data/cheditor4/1203/7271aa94af5e772efaa76d88cf0f0cbd_ouQcXvIJAe8F9YEW.gif)
![](http://vic21mr.cafe24.com/data/cheditor4/1203/7271aa94af5e772efaa76d88cf0f0cbd_NTEtzSxvHnIjNW43ilq.gif)
![](http://vic21mr.cafe24.com/data/cheditor4/1203/7271aa94af5e772efaa76d88cf0f0cbd_jEUhlNmYihEVyonopk4cvIs4z47Ll.gif)
![](http://vic21mr.cafe24.com/data/cheditor4/1203/7271aa94af5e772efaa76d88cf0f0cbd_2gepnJ6SVhpMig1.gif)
![](http://vic21mr.cafe24.com/data/cheditor4/1203/7271aa94af5e772efaa76d88cf0f0cbd_MWY82Oh1YYt8vJHx5cHeDKtvKxlrl4u.gif)
![](http://vic21mr.cafe24.com/data/cheditor4/1203/7271aa94af5e772efaa76d88cf0f0cbd_lpYozrXthvqFcgIDB.gif)
![](http://vic21mr.cafe24.com/data/cheditor4/1203/7271aa94af5e772efaa76d88cf0f0cbd_tyJQwdlp2xJdnmj7Dutuq1eBG6sK.gif)
Selection of CHROMABOND¢ç phases for SPE
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Phase selection for water-soluble, non-ionic samples
Sample solvent |
Sample polarity |
Phases recommended for adsorption |
Solvents recommended for elution (selection) |
aqueous |
nonpolar |
RP phases |
HR-X, Easy, HR-P |
hexane, dichloromethane |
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C18 ec, C18, C18 Hydra |
acetonitrile, alcohols |
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C8, C4, C2 |
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C6H11 ec, C6H5 |
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NP phases |
CN |
trichloromethane, dichloromethane |
aqueous |
moderately polar |
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SiOH |
ethyl acetate, alcohols, water |
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NH2 |
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aqueous |
polar |
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CN, OH (Diol) |
as above |
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NH2 |
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Phase selection for water-soluble, ionic samples
Sample solvent |
Sample polarity |
Phases recommended for adsorption |
Solvents recommended for elution (selection) |
aqueous |
cationic |
Ion exchange phases |
HR-XC, HR-XCW, PS-H+ |
acids, salt solutions, buffers |
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SA, PCA, PSA |
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aqueous |
anionic |
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HR-XA, HR-XAW, PS-OH– |
acids, salt solutions, buffers |
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SB |
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NP phases |
NH2 |
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Phase selection for samples soluble in organic solvents
Sample solvent |
Sample polarity |
Phases recommended for adsorption |
Solvents recommended for elution (selection) |
aqueous |
nonpolar |
RP phases |
HR-X, Easy, HR-P |
hexane, dichloromethane |
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C18 ec, C18, C18 Hydra |
acetonitrile, alcohols |
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C8, C4, C2 |
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C6H11 ec, C6H5 |
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PS-RP |
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NP phases |
CN |
chloroform, dichloromethane |
organic |
moderately polar |
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SiOH |
ethyl acetate, alcohols, water |
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NH2 |
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organic |
polar |
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CN, OH (Diol)
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as above |
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NH2 |
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Copyright 2012 by MACHEREY-NAGEL GmbH & Co. KG
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