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Watchers HPLC column by ISUIND
õ¿¬¹° ºÐ¸® Á¤Á¦ ´Ü¹éÁú PROTEIN ÆéŸÀ̵å PEPTIDE ¾Æ¹Ì³ë»ê AMINOSAN À̼ö»ê¾÷ SPE SYRING FILTER TLC HPLC MPLC PREPLC FLASH CPC MACHEREY NAGEL INTERCHIM ARMEN WATHCERS ISUIND C18 C8 C4 C2 ODS NH2 Florisil aluminum oxide SAC SCX LH-20 SILICA DAC PREP CARTRIDGE TLC cutter watchers column
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ÃÑ °Ô½Ã¹° 0°Ç, ÃÖ±Ù 0 °Ç
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NUCLEODUR¢ç high purity silica phases for HPLC |
Features |
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NUCLEODUR¢ç °íµµÀÇ ¹°¸®Àû Ư¼ºÀ» Á¦°øÇÏ´Â ¿Ïº®ÇÑ ÇÕ¼º BŸÀÔ ½Ç¸®Ä«
(Á¦ 3¼¼´ëÀÇ ½Ç¸®Ä«)
¿ÏÀü ±¸Çü ÀÔÀÚÀÇ ÇüÅÂ
¿ì¼öÇÑ ¹Ì¼¼ Ç¥¸é
³ôÀº ¾Ð·ÂÀÇ ¾ÈÀü¼º
³·Àº ±Ý¼Ó ÇÔ·®
NUCLEODUR¢ç´Â ÃÖ÷´Ü ½Ç¸®Ä«·Î Çö´ë HPLCÀÇ ´Ü°è¸¦ ÀÌ»óÀûÀ¸·Î ¿Ã·Á ÁÙ
ÀÖ´Â ¹°ÁúÀÔ´Ï´Ù. |
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ºÐ¸®´ÉÀ» Áõ°¡½ÃÅ°±â À§ÇÑ ¹æ¹ý NUCLEODUR¢ç with 1.8 µm particle size |
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Summary of available NUCLEODUR¢ç phases
Phase |
Modification |
% C |
pH range |
Particle sizes [µm] |
USP |
Structure |
C18 Gravity |
octadecyl, high density coating, multi-endcapping |
18 |
1–11 |
1.8 ¡¤ 3 ¡¤ 5 |
L1 |
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C8 Gravity |
octyl, high density coating, multi-endcapping |
11 |
1–11 |
1.8 ¡¤ 5 |
L7 |
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C18 Isis |
octadecyl, specially cross-linked modification, endcapped |
20 |
1–10 |
1.8 ¡¤ 3 ¡¤ 5 |
L1 |
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C18 Pyramid |
octadecyl with polar endcapping |
14 |
1–9 |
1.8 ¡¤ 3 ¡¤ 5 |
L1 |
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PolarTec |
octadecyl with embedded polar group |
17 |
1–9 |
3 ¡¤ 5 |
L1 L60 |
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PFP |
pentafluorophenyl-propyl with multi-endcapping |
8 |
1–9 |
3 ¡¤ 5 |
L43 |
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Sphinx RP |
bifunctional, balanced ratio of propylphenyl and octadecyl, endcapping |
15 |
1–10 |
1.8 ¡¤ 3 ¡¤ 5 |
L1 L11 |
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C18 HTec |
octadecyl, high density coating, high capacity, multi-endcapping |
18 |
1–11 |
1.8 ¡¤ 3 ¡¤ 5 7 ¡¤ 10 |
L1 |
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C18 ec |
octadecyl, medium density, endcapping |
17.5 |
1–9 |
3 ¡¤ 5 and larger |
L1 |
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C8 ec |
octyl, medium density, endcapping |
10.5 |
1–9 |
3 ¡¤ 5 |
L7 |
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HILIC |
ammonium – sulfonic acid |
7 |
2–8.5 |
1.8 ¡¤ 3 ¡¤ 5 |
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CN/CN-RP |
cyano (nitrile) for NP and RP separations |
7 |
1–8 |
3 ¡¤ 5 |
L10 |
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NH2/NH2-RP |
aminopropyl for NP and RP separations |
2.5 |
2–8 |
3 ¡¤ 5 |
L8 |
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SiOH |
unmodified NUCLEODUR¢ç |
– |
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3 ¡¤ 5 and larger |
L3 |
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NUCLEOSHELL core-shell silica phases for HPLC |
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Features |
Summary of available NUCLEOSHELL phases
Phase |
Modification |
% C |
pH range |
Particle size [µm] |
USP |
Structure |
RP 18 |
Octadecyl, multi-endcapping |
7.5 |
1–11 |
2.7 |
L1 |
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PFP |
Pentafluorophenyl, multi-endcapping |
~ 3 |
1–9 |
2.7 |
L43 |
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HILIC |
Ammonium – sulfonic acid |
1.3 |
2–8.5 |
2.7 |
– |
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HPLC ºÐ¸®¿¡ ´ëÇÑ ¿ä±¸´Â Áö¼ÓÀûÀ¸·Î ºÐ¸® È¿À² ¹× °ËÃâ ÇÑ°è»Ó¸¸ ¾Æ´Ï¶ó, °¢°¢ÀÇ ºÐ¼®À» À§ÇÑ ½Ã°£ÀÌ ¿ä±¸ »çÇ׿¡ °ü·ÃÇÏ¿© Áõ°¡ÇÏ°í ÀÖ½À´Ï´Ù. ÄÚ¾î - ½© ±â¼ú Àº ¿¬±¸¿Í Ç°Áú °ü¸®ÀÇ È¿À²¼º Áõ´ë¸¦ À§ÇÑ »õ·Î¿î Ç¥ÁØÀ» ¼³Á¤ÇÕ´Ï´Ù.
Benefits of core-shell technology |
Core-shell particles vs. totally porous silica gel |
ªÀº È®»ê °æ·Î |
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ºü¸¥ ¹°Áú Àü´Þ(C term of the Van Deemter equation) |
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LC¿¡¼ ³ôÀº À¯¼Ó¿¡¼µµ ÇÇÅ© È®´ë°¡ ¾ø´Ù. |
Á¼Àº ÀÔÀÚ Å©±â ºÐÆ÷ (d90/d10 ~ 1.1) |
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¾ÈÁ¤ÀûÀÎ ÆÐÅ· |
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Column efficiency of NUCLEOSHELL ~ 250000/m (HETP ~ 4 µm) |
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Minimized fractional heat |
Resolution Rs as function of particle size |
Columns: |
50 x 4 mm NUCLEOSHELL RP 18, 2.7 µm NUCLEODUR¢ç C18 Gravity, 3 µm NUCLEODUR¢ç C18 Gravity, 1.8 µm |
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Eluent: Flow rate: Temperature: Detection: |
acetonitrile – water (60:40, v/v) 1 mL/min 25 ¡ÆC UV, 254 nm |
Peaks: 1. Naphthalene 2. Ethylbenzene |
Better resolution at lower back pressure and shorter retention time |
NUCLEOSIL¢ç standard silica phases for HPLC
Features
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NUCLEOSIL¢ç ¼øµµ°¡ ³ô°í ±ÕÀÏÇÑ SIO2 ±¸Á¶·Î ¿Ïº®ÇÑ ´Ù°ø¼º ±¸Çü ½Ç¸®Ä«
Å©·Î¸¶Åä±×·¡ÇÇ ºÐ¾ß¿¡¼ ÀϹÝÀûÀ¸·Î »ç¿ëµÇ´Â ½Ç¸®Ä«
HPLCºÐ¾ß¿¡¼ ÃÖÃÊ·Î »ç¿ëµÈ ±¸Çü ½Ç¸®Ä«
70³â´ë¿¡ °³¹ßµÇ¾î Áö±Ýµµ ¼¼°èÀûÀ¸·Î »ç¿ëµÇ´Â HPLC ÆÐÅ·¿ë ½Ç¸®Ä«
HPLC¿¡¼ ½Å·ÚÇÒ ¼ö ÀÖ´Â Á¦Ç°
HPLC¿¡¼ °¡Àå ´Ù¾çÇÏ°Ô »ç¿ëÇÒ ¼ö ÀÖ´Â ½Ç¸®Ä« |
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Summary of available NUCLEOSIL¢ç phases
Phase |
Modification |
Formula |
RP phases |
NUCLEOSIL¢ç C18 |
medium density octadecyl, endcapped |
-(CH2)17-CH3 |
NUCLEOSIL¢ç C18 AB |
crosslinked octadecyl, endcapped, high steric selectivity |
-(CH2)17-CH3 |
NUCLEOSIL¢ç C18 HD |
high density octadecyl, endcapped |
-(CH2)17-CH3 |
NUCLEOSIL¢ç C18 Nautilus |
hydrophilic octadecyl, embedded polar group, endcapped |
-(CH2)17-CH3 |
NUCLEOSIL¢ç Protect I |
special RP phase with protective polar group, endcapped |
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NUCLEOSIL¢ç C8 ec |
medium density octyl, endcapped |
-(CH2)7-CH3 |
NUCLEOSIL¢ç C8 |
standard octyl, not endcapped |
-(CH2)7-CH3 |
NUCLEOSIL¢ç C8 HD |
high density octyl, endcapped |
-(CH2)7-CH3 |
NUCLEOSIL¢ç C4 |
medium density butyl, endcapped |
-(CH2)3-CH3 |
NUCLEOSIL¢ç C2 |
dimethyl |
-(CH3)2 |
NUCLEOSIL¢ç C6H5 |
standard phenyl, not endcapped |
-(CH2)3-C6H5 |
Polar phases |
NUCLEOSIL¢ç CN |
cyanopropyl (nitrile) |
-(CH2)3-CN |
NUCLEOSIL¢ç OH |
diol (dihydroxypropyl) |
-(CH2)3-O-CH2-CH(OH)-CH2OH |
NUCLEOSIL¢ç NH2 |
aminopropyl |
-(CH2)3-NH2 |
NUCLEOSIL¢ç N(CH3)2 |
dimethylamino |
-(CH2)3-N(CH3)2 |
NUCLEOSIL¢ç SA |
benzenesulfonic acid, strongly acidic cation exchanger (SCX) |
-(CH2)3-C6H4-SO3Na |
NUCLEOSIL¢ç SB |
quaternary ammonium groups, strongly basic anion exchanger (SAX) |
-(CH2)3-C6H4-CH2-N+(CH3)3Cl– |
NUCLEOSIL¢ç SiOH |
unmodified |
(SiO2)n |
HPLC phases for special applications |
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Summary |
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¡ØMACHERY-NAGEL HOMEPAGE
Copyright 2012 by MACHEREY-NAGEL GmbH & Co. KG
õ¿¬¹° ºÐ¸® Á¤Á¦ ´Ü¹éÁú PROTEIN ÆéŸÀ̵å PEPTIDE ¾Æ¹Ì³ë»ê AMINOSAN À̼ö»ê¾÷ SPE SYRING FILTER TLC HPLC MPLC PREPLC FLASH CPC MACHEREY NAGEL INTERCHIM ARMEN WATHCERS ISUIND C18 C8 C4 C2 ODS NH2 Florisil aluminum oxide SAC SCX LH-20 SILICA DAC PREP CARTRIDGE TLC cutter watchers column
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